▎ 摘　　要

Aim: The development of new and efficient antibacterial agents is urgent to overcome emerging antimicrobial resistance. Materials & methods: Herein, we have presented a new type of 3D antibacterial system to prompt bacteria to contact with the any plane of nanocomposites. Results: Comparing the antibacterial activity of graphene oxide, reduced graphene oxide and graphene-loaded nanoflower-like nickelous hydroxide (GN/Ni(OH)(2)) nanocomposites; the GN/Ni(OH)(2) showed stronger bactericidal capability toward Gram-negative/-positive bacteria. Moreover, the GN/Ni(OH)(2) with low cytotoxicity can promote it as 'green' antimicrobial agents. And, the GN/Ni(OH)(2) presented long-term stable antibacterial effectiveness after 2-month storage. The antibacterial mechanisms of GN/Ni(OH)(2) were evidenced as the 3D contact and violent damage to the bacterial structure. Conclusion: The GN/Ni(OH)(2) provides new insights into the antibacterial properties of 3D nanocomposites for effectively fighting pathogen threats in biomedicine and public health.