• 文献标题:   Impedimetric Dengue Biosensor based on Functionalized Graphene Oxide Wrapped Silica Particles
  • 文献类型:   Article
  • 作  者:   JIN SA, POUDYAL S, MARINERO EE, KUHN RJ, STANCIU LA
  • 作者关键词:   graphene, dengue, rna hybridization, impedance biosensor
  • 出版物名称:   ELECTROCHIMICA ACTA
  • ISSN:   0013-4686 EI 1873-3859
  • 通讯作者地址:   Purdue Univ
  • 被引频次:   15
  • DOI:   10.1016/j.electacta.2016.02.116
  • 出版年:   2016

▎ 摘  要

A composite of 3-Aminopropyltriethoxysilane (APTES) functionalized graphene oxide (APTES-GO) wrapped on SiO2 particles (SiO2@APTES-GO) was prepared via self-assembly. Transmission electron microscopy (TEM) and ATR-Fourier Transform Infrared spectroscopy (ATR-FTIR) confirmed wrapping of the SiO2 particles by the APTES-GO sheets. An impedimetric biosensor was constructed and used to sensitively detect dengue DNA and dengue RNA via primer hybridization using different oligonucleotide sequences. The results demonstrated that the SiO2@APTES-GO electrode material led to enhanced dengue RNA detection sensitivity with selectivity and detection limit (1 femto-Molar), compared to both APTES-GO and APTES-SiO2. The three-dimensional structure, higher contact area, electrical properties and the ability for rapid hybridization offered by the SiO2@APTES-GO led to the successful design of a dengue biosensor with the lowest detection limit reported to date. (C) 2016 Elsevier Ltd. All rights reserved.