▎ 摘　　要

Graphene oxide (GO) nanoparticles based electrochemical nanobiosensor was developed to check the sensitivity response of biological elements. Present work is related to synthesize GO nanoparticles by Hummers method for the detection of glucose using electrochemical nanobiosensor. Two dimensional hexagonal crystal structure and crystallite size was calculated by XRD analysis and randomly crumpled like surface morphology was identified by using SEM micrograph. Furthermore, different rotational and vibrational functional groups (C-O, C=C, CO2 and C-H) attached to the layer of graphene oxide and peak shift toward longer wavelength was studied by FTIR and UV-visible spectrum. Finally, voltammetry cyclic used for sensitivity of glucose and presence of glucose was recorded in concentration of 5mM at scan rate of 50mVs(-1). In future this type of nanobiosensor used for the detection of biological element like DNA.