▎ 摘　　要

A modified, selective; highly sensitive and accurate procedure for the determination of trace amounts of manganese and iron ions is established in the presented work. 3-(1-Methyl-1H-pyrrol-2-yl)-1H-pyrazole-5-carboxylic add (MPPC) and graphene oxide (GO) were used in a glass column as chelating reagent and as adsorbent respectively prior to their determination by flame atomic absorption spectrometry. The adsorption mechanism of titled metals complexes on GO was investigated by using computational chemistry approach based on PM6 semi-empirical potential energy surface (PES). The effect of some parameters including pH, flow rate and volume of sample and type, volume and concentration of eluent, as well as the adsorption capacity of matrix ions on the recovery of Mn(II) and Fe(III) was investigated. The limit of detection was 145 and 162 ng L-1 for Mn(II) and Fe(III), respectively. Calibration was linear over the range of 0.31-355 mu g L-1 for Mn(II) and 0.34-380 mu g L-1 for Fe(III) ions. The method was successfully applied for the determination of understudied ions in water, food and biological samples. (C) 2013 Elsevier B.V. All rights reserved.