▎ 摘　　要

The high cost and low reusability of natural enzymes greatly limit their application in biosensing. In this work, a sustainable nanozyme with light-driven oxidase-like activity was fabricated by integrating protein-capped silver nanoclusters (AgNCs) with graphene oxide (GO) through multiple non-covalent interactions. The prepared AgNCs/GO nanozyme could effectively catalyze the oxidation of various chromogenic substrates by activating dissolved O2 to reactive oxygen species under visible light irradiation. Moreover, the oxidase-like activity of AgNCs/GO could be well controlled by switching on and off the visible light source. Compared with natural peroxidase and most of other oxidase-mimicking nanozymes, AgNCs/GO possessed improved catalytic activity owing to the synergistic effect between AgNCs and GO. More importantly, AgNCs/GO showed outstanding stability against precipitation, pH (2.0-8.0), temperature (10-80 degrees C), and storage and could be reused at least 6 cycles without obvious loss in catalytic activity. On this basis, AgNCs/GO nanozyme was used to develop a colorimetric assay for the determination of total antioxidant capacity in human serum, which had the merits of high sensitivity, low cost, and good safety. This work holds a promising prospect in developing sustainable nanozymes for biosensing and clinical diagnosis.