• 文献标题:   Amperometric urea biosensors based on sulfonated graphene/polyaniline nanocomposite
  • 文献类型:   Article
  • 作  者:   DAS G, YOON HH
  • 作者关键词:   electrochemical deposition, sulfonated graphene oxide, urease
  • 出版物名称:   INTERNATIONAL JOURNAL OF NANOMEDICINE
  • ISSN:   1178-2013
  • 通讯作者地址:   Gachon Univ
  • 被引频次:   22
  • DOI:   10.2147/IJN.S88315
  • 出版年:   2015

▎ 摘  要

An electrochemical biosensor based on sulfonated graphene/polyaniline nanocomposite was developed for urea analysis. Oxidative polymerization of aniline in the presence of sulfonated graphene oxide was carried out by electrochemical methods in an aqueous environment. The structural properties of the nanocomposite were characterized by Fourier-transform infrared, Raman spectroscopy, X-ray photoelectron spectroscopy, and scanning electron microscopy techniques. The urease enzyme-immobilized sulfonated graphene/polyaniline nanocomposite film showed impressive performance in the electroanalytical detection of urea with a detection limit of 0.050 mM and a sensitivity of 0.85 mu A.cm(-2).mM(-1). The biosensor achieved a broad linear range of detection (0.12-12.3 mM) with a notable response time of approximately 5 seconds. Moreover, the fabricated biosensor retained 81% of its initial activity (based on sensitivity) after 15 days of storage at 4 degrees C. The ease of fabrication coupled with the low cost and good electrochemical performance of this system holds potential for the development of solid-state biosensors for urea detection.