▎ 摘　　要

Breast cancer is the most prevalent type of cancer in women; hence, many researches have been focused on developing effective treatment protocols. In this study, a novel nanocarrier was fabricated for gene and photoothermal combination cancer therapy by conjugating histone methyltransferase complex subunit SET1 (hSET1) on reduced polydopamine coated graphene oxide nanosheets (rGO-PDA). The rGO-PDA nanocarriers provide higher near-infrared absorption and further integrating with hSET1 antisense as an anticancer gene that down-regulates the amount of hSET1 overexpressed and suppresses the proliferation of cancer cells. The nanoplatform was prepared by polymerizing of dopamine, a mussel adhesive protein, on graphene oxide nanosheets in alkaline media, followed by conjugation of hSET1 antisense on rGO-PDA nanocarriers which was examined by gel retardation assay. After Laser irradiation, the amount of hSET1 was estimated in overexpressed cells treated with this formulation by real-time reverse transcription-polymerase chain reaction (RT-PCR). The size and zeta potential of prepared nanocarriers were 140 nm and 29.4 mv respectively. In vitro cellular tests confirmed that the prepared nanoplatform was biocompatible with no cell toxicity. The nanocarrier as a photothermal agent generating hyperthermia, led to a better release of hSET1 antisense under NIR (nearinfrared) and more apoptosis of cancer cells and RT-PCR implied a considerable decrease of hSET1 expression. Outstanding photothermal conversion and suitable potential of rGO-PDA for interaction with oligonucleotides is expected as a suitable system for gene delivery and photoresponsive therapy of solid tumors simultaneously.