• 文献标题:   Structural-functional analysis of engineered protein-nanoparticle assemblies using graphene microelectrodes
  • 文献类型:   Article
  • 作  者:   PING JL, PULSIPHER KW, VISHNUBHOTLA R, VILLEGAS JA, HICKS TL, HONIG S, SAVEN JG, DMOCHOWSKI IJ, JOHNSON ATC
  • 作者关键词:  
  • 出版物名称:   CHEMICAL SCIENCE
  • ISSN:   2041-6520 EI 2041-6539
  • 通讯作者地址:   Univ Penn
  • 被引频次:   3
  • DOI:   10.1039/c7sc01565h
  • 出版年:   2017

▎ 摘  要

The characterization of protein-nanoparticle assemblies in solution remains a challenge. We demonstrate a technique based on a graphene microelectrode for structural-functional analysis of model systems composed of nanoparticles enclosed in open-pore and closed-pore ferritin molecules. The method readily resolves the difference in accessibility of the enclosed nanoparticle for charge transfer and offers the prospect for quantitative analysis of pore-mediated transport, while shedding light on the spatial orientation of the protein subunits on the nanoparticle surface, faster and with higher sensitivity than conventional catalysis methods.