▎ 摘　　要

Nanocomposite-modified structures based on reduced graphene oxide (rGO) are preferred because of their excellent advantages. Here we assessed the comparative interaction of graphene oxide (GO), chem-ically reduced GO (rGO), copper and nickel spinel ferrite nanoparticles (CuFe2O4 NPs and NiFe2O4 NPs) and their nanocomposites with reduced graphene oxide (rGO -CuFe2O4, rGO -NiFe2O4 NCs). The synthe-sized nanomaterials were characterized by X-ray diffraction (XRD) and transmission electron microscope (TEM) analysis. The cytotoxic analysis revealed a nontoxic behavior at concentrations below 50 mu g/mL in human fetal lung fibroblast (MRC-5), human hepatocellular carcinoma (HepG2), and human glioblastoma (TG98) cell lines. The IC50 values were found to be lower in rGO than that of GO in MRC-5 and T98G cell lines. 50 mu g/mL and higher concentrations of rGO -CuFe2O4 NCs displayed more potent cytotoxic effects in MRC-5 and HepG2 cell lines compared with the CuFe2O4 NPs. Cytotoxicity was decreased in HepG2 and T98G cell lines treated with rGO -NiFe2O4 NCs compared to NiFe2O4 NPs but increased in MRC-5. According to the molecular docking results, DNA binding modes of all nanomaterials were found to be minor groove recognition, while their DNA binding affinities ranged between-4.78 and-12.89 kcal/mol. Docking results showed that, based on their sharp-edged conformations, GO and rGO, and rGO-MFe2O4 (M: Cu, Ni) composites may be promising shape-selective DNA minor groove binders, with the potential to cause specific DNA damage in cancer cells. (c) 2023 Elsevier B.V. All rights reserved.