• 文献标题:   Low-level expression of purine bases in BALB/3T3 cells monitored by ultrasensitive graphene-based glass carbon electrode
  • 文献类型:   Article
  • 作  者:   GAO GG, XU GB, LI JL, CUI JW, WU DM, QIU HB
  • 作者关键词:   graphene, ionic liquid, purine, electrochemical, cell
  • 出版物名称:   ANALYTICAL BIOCHEMISTRY
  • ISSN:   0003-2697 EI 1096-0309
  • 通讯作者地址:   Jiamusi Univ
  • 被引频次:   10
  • DOI:   10.1016/j.ab.2014.08.034
  • 出版年:   2014

▎ 摘  要

Using an ultrasensitive chemically reduced graphene oxide and ionic liquid modified glass carbon electrode (RGI-GCE), separated electrochemical signals of adenine and hypoxanthine in both human breast cancer (MCF-7) and mouse embryonic fibroblast (BALB/3T3) cells were observed. For the first time, low-level expression of purine bases in noncancerous BALB/3T3 cells can be electrochemically monitored. The metabolism of purine bases in carcinogen agent-contaminated BALB/3T3 cells was also investigated through the change of electrochemical signals ascribed to different purine bases, which opens a new electrochemical approach to the exploration of a low-level purine mechanism in noncancerous cells. (C) 2014 Elsevier Inc. All rights reserved.