▎ 摘 要
Graphene oxide nanosheets was prepared, modification with 3-aminopropyltriethoxysilane and used as the carrier for immobilization of NADH oxidase (NOX) from Streptococcus mutans. The prepared nanosheets were characterized with FTIR. A high immobilization yield of 92% and activity recovery of 91% were achieved at pH 7.0 and 25 degrees C. The thermostability of the immobilized NOX was improved about 60% compared with the free enzyme. And the immobilized NOX preserved about 92% of its initial activity after 500 h storage at 4 degrees C, while free NOX was totally denatured after 200 h at the same conditions. These results indicate that NH2-functionalized graphene oxide nanosheets has a good potential as carrier for immobilization of proteins, especially enzymes.