• 文献标题:   Preparation of NH2-Functionalized Graphene Oxide Nanosheets for Immobilization of NADH Oxidase
  • 文献类型:   Article
  • 作  者:   GU XY, PENG JX
  • 作者关键词:   graphene oxide, nanosheet, immobilization, nadh oxidase, stability
  • 出版物名称:   JOURNAL OF NANOSCIENCE NANOTECHNOLOGY
  • ISSN:   1533-4880 EI 1533-4899
  • 通讯作者地址:   Jiangsu Univ Sci Technol
  • 被引频次:   2
  • DOI:   10.1166/jnn.2018.15212
  • 出版年:   2018

▎ 摘  要

Graphene oxide nanosheets was prepared, modification with 3-aminopropyltriethoxysilane and used as the carrier for immobilization of NADH oxidase (NOX) from Streptococcus mutans. The prepared nanosheets were characterized with FTIR. A high immobilization yield of 92% and activity recovery of 91% were achieved at pH 7.0 and 25 degrees C. The thermostability of the immobilized NOX was improved about 60% compared with the free enzyme. And the immobilized NOX preserved about 92% of its initial activity after 500 h storage at 4 degrees C, while free NOX was totally denatured after 200 h at the same conditions. These results indicate that NH2-functionalized graphene oxide nanosheets has a good potential as carrier for immobilization of proteins, especially enzymes.