▎ 摘　　要

Herein we report a NADH sensor platform based on the gold nanoparticles (AuNPs) decorated electrochemically reduced graphene oxide (ERGO) stabilized with poly( allyamine hydrochloride) (PAH). The ERGO was obtained by direct electrochemical reduction of graphene oxide onto the electrode surface. The electrode material was characterized by Raman spectroscopy, Scanning Electron Microscopy and UV-vis spectrometry. The composite AuNPs-ERGO-PAH promotes the electron transfer between NADH and the modified screen-printed electrode surface at +510 mV with a high sensitivity of 131.0 +/- 1.2 mu A mM(-1) cm(2) and a detection limit of 3.5 mu M in amperometric measurements. The differential pulse voltammetry allowed the selective NADH detection in the presence of ascorbic acid, dopamine, glucose and uric acid. The AuNPs-ERGO-PAH/SPE sensing platform represents a promising starting point for development of dehydrogenase-based biosensors. (C) 2015 Elsevier BY. All rights reserved.