▎ 摘　　要

The utilization of green syntheses is important for reducing environmental pollution. Many synthetic approaches have been developed for the synthesis of isatin-thiazolidine hybrids. However, none of these methods directly address a green methodology for the synthesis of this key organic scaffold. This work deals with the synthesis of isatin-thiazolidine hybrids using graphene oxide (GO) catalyst in deep eutectic solvent (DES) as a green media. Synthesized compounds were evaluated for in -vitro antibacterial, anticancer and cytotoxic activities. The tested compounds exhibited more antibacterial activity against Gram-positive bacteria than Gram-negative bacteria. The compounds were observed to contain electron -withdrawing groups which displayed more bacterial inhibi- tion. In summary, the current study reports that eco-friendly graphene oxide together with deep eutectic solvent catalyzed the synthesis of isatin-thiazolidine hybrids, whereby a high product yield was achieved in a short space of time. Although, newly synthesized compounds could not produce efficient biological activity, cytotoxicity instead.