▎ 摘　　要

It is of great significance for human health and environmental monitoring to establish an ultrasensitive assay method for Hg2+. In this work, a label-free and highly sensitive electrochemiluminescence (ECL) aptasensor was developed for the sensitive and selective detection of Hg2+ without additional coreactants. Poly (aniline-luminol)/graphene oxide (P(ANi-Lu)/GO) nanocomposites were prepared, and chitosan (CS) was used to modify the surface of the material positively charged. The prepared P(ANi-Lu)/GO/CS was characterized by scanning electron microscopy (TEM), UV-Vis absorption spectra, fluorescence spectra and electrochemical impedance spectroscopy. The P(ANi-Lu)/GO/CS exhibited a stable and excellent electrochemiluminescence (ECL) property. Aptamer was adsorbed on P(ANi-Lu)/GO/CS sensing interface through electrostatic interaction, and Hg2+ was detected using T-Hg2+-T configuration as ECL intensity controller. The results showed that Hg2+ can be quantitatively determined in the range of 1.0 x 10(-15) similar to 1.0 x 10(-7) M with a detection limit of 4.8 x 10(-16) M.