• 文献标题:   A sensitive electrochemical biosensor for specific DNA sequence detection based on flower-like VS2, graphene and Au nanoparticles signal amplification
  • 文献类型:   Article
  • 作  者:   FANG LX, CAO JT, HUANG KJ
  • 作者关键词:   flowerlike vs2, avian influenza virus h5n1 gene sequence, electrochemical sensor, signal amplification
  • 出版物名称:   JOURNAL OF ELECTROANALYTICAL CHEMISTRY
  • ISSN:   1572-6657 EI 1873-2569
  • 通讯作者地址:   Xinyang Normal Univ
  • 被引频次:   16
  • DOI:   10.1016/j.jelechem.2015.03.026
  • 出版年:   2015

▎ 摘  要

Flower-like VS2 was prepared by a simple one-step hydrothermal strategy. A highly sensitive and label-free electrochemical aptasensor was developed by employing self-assembly of DNA aptamers on flower-like VS2, graphene and Au nanoparticles (AuNPs)-modified glassy carbon electrodes. Avian influenza virus H5N1 gene sequence was chosen as the target DNA and [Fe(CN)(6)](3-/4-) solution was used as the electrochemical indicator. AuNPs were coated onto VS2-graphene modified electrode and used as a platform for the immobilization of the aptamers. This VS2-graphene composite offered a nanostructure with a large effective surface area, highly electrocatalytic activities and electronic conductivity. Therefore, the amount of aptamer immobilized onto the electrode was increased while the accessibility of the detection target was maintained. The decrease in the peak current was linear with the logarithm of the concentration of the H5N1 specific sequence from 5.0 x 10(-13) to 5.0 x 10(-10) M (R = 0.9912) with a detection limit of 5.2 x 10(-14) M. The proposed method also showed acceptable stability and reproducibility. This work provides a promising immobilization strategy for the fabrication of biosensors and offers an alternative and sensitive method for DNA detection. (C) 2015 Elsevier B.V. All rights reserved.