▎ 摘　　要

Nanoparticles of noble metals are well known to display unique optical properties due to the localized surface plasmon resonance (LSPR) phenomenon, making them applicable for use as transducers in various nanoplasmonic sensor configurations. In order to develop a sensor chip, Au nanoparticles (AuNPs) were decorated onto a substrate using a self-assembly monolayer method. The optimized substrate was subsequently prepared by functionalization with (3-Aminopropyl) triethoxysilane (APTES), while also being immersed into colloidal AuNPs. Using a uniform layer of graphene oxide (GO) and reduced GO (rGO), the AuNPs/GO and rGO hybrid structures were employed along with an appropriate optical setup acting as a smart sensor chip for detection of different concentrations of biomaterials. The optimum sensor chip (0.5% APTES; 12 h immersion time) resulted in a chip with 29% absorption and sharper plasmon peak. This appropriate condition remained constant after adding rGO, indicating that Glass/AuNPs/rGO chip will be suitable for sensory applications and detection of biomolecules.