▎ 摘　　要

An electrochemical method based on a directly electrochemically reduced graphene oxide (ERGO) film coated on a glassy carbon electrode (GCE) was developed for the rapid and convenient determination of rutin in plasma. ERGO was modified on the surface of GCE by one-step electro-deposition method. Electrochemical behavior of rutin on ERGO/GCE indicated that rutin underwent a surface-controlled quasi-reversible process and the electrochemical parameters such as charge transfer coefficient (a), electron transfer number (n) and electrode reaction standard rate constant (k(s)) were 0.53.2 and 3.4s(-1) respectively. The electrochemical sensor for rutin in plasma provided a wide linear response range of 4.70 x 10(-7)-1.25 x 10(-5) M with the detection limit (s/n =3) of 1.84 x 10(-8) M. The assay was successfully used to the pharmacokinetic study of rutin. The pharmacokinetic parameters such as elimination rate half-life (t(1/2)), area under curve (AUC), and plasma clearance (CL) were calculated to be 3.345 +/- 0.647 min, 5750 +/- 656.0 mu g min/mL, and 5.891 +/- 0.458 mL/min/kg, respectively. The proposed method utilized a small sample volume of 10 tit and had no complicated sample pretreatment (without deproteinization), which was simple, eco-friendly, and time- and cost-efficient for rutin pharmacokinetic studies. (C) 2015 Xi'an Jiaotong University. Production and hosting by Elsevier B.V.