▎ 摘　　要

A novel platform for electrocatalysis of isoniazid (INH) based on 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) and reduced graphene oxide (rGO) immobilized in a Nafion membrane was developed. The composite (DDQ/rGO) was characterized by Fourier-transform infrared spectroscopy (FTIR) and the sensor response for the analyte was studied by cyclic voltammetry (CV) and chronoamperometry techniques. The DDQ/rGO/Nafion (R) modified electrode showed an excellent electrocatalytic activity toward INH oxidation with a decrease of the overpotential of about 660 mV vs Ag/AgCl, compared to the process at an unmodified electrode. Under optimized conditions, the sensor showed a wide linear range for isoniazid from 0.5 up to 380 mu mol L-1 with r(2) of 0.999. The sensor was applied for the INH determination in drug samples utilized in the tuberculosis treatment and the results were satisfactory. The detection limit, repeatability, long-term stability, time of response and linear response range were also investigated. (C) 2016 Elsevier B.V. All rights reserved.