▎ 摘　　要

Simultaneous enhancement of osseointegration and bacterial infection prohibition through surface modifications is a challenging but promising approach to achieve durable implantation. To that end, we present a multifunctional surface coating composed of graphene nanolayers and hierarchical well-aligned TiO2 nanotubes with a nanoporous top layer (cRTNT). FESEM studies reveal tunable increasing island morphologies of graphene nanolayers (G) on cRTNT by a cyclic voltammetry process. XPS analysis shows that the enhanced interface chemistry is due to TiO2-carbon bonding. The roughness of the sample containing a medium amount of graphene, cRTNT-75%GO, was calculated similar to 289 nm, which was 543% higher than the Ti6Al4V surface. ALP activity increases by more than 25% for cRTNT/GO samples compared to pure cRTNT. Fluorescent microscopy indicates an approximately 92% decrease in bacterial colony formation and up to 85% improvement in the number of bacteria per field on cRTNT with increasing graphene amount compared to the Ti-6Al-4V surface. Although the increasing amount of graphene up to 95% shows better antibacterial performance, it does not provide a proper surface for adhesion and growth of the bone cells. According to all bio-assays, the nanocomposite coating with a medium amount of graphene (cRTNT-75%G) is proposed as the best sample in terms of biological properties, including improving cell behavior and preventing infection.