• 文献标题:   A cationic conjugated polymer and graphene oxide: Application to amplified fluorescence detection of sinapine
  • 文献类型:   Article
  • 作  者:   ZHANG Z, XIANG X, SHI JB, HUANG FH, XIA XY, ZHENG MM, HAN L, TANG H
  • 作者关键词:   sp, go, pfp, fret, fluorescence amplification
  • 出版物名称:   SPECTROCHIMICA ACTA PART AMOLECULAR BIOMOLECULAR SPECTROSCOPY
  • ISSN:   1386-1425
  • 通讯作者地址:   Chinese Acad Agr Sci
  • 被引频次:   0
  • DOI:   10.1016/j.saa.2018.05.110
  • 出版年:   2018

▎ 摘  要

An amplified fluorescence strategy is described for the detection of sinapine (SP) by using a cationic conjugated polymer (PFP) and graphene oxide (GO). It is observed that the fluorescein (FAM)-labeled single-stranded DNA (FAM-DNA) is absorbed on the surface of GO if SP is absent. This causes that fluorescence resonance energy transfer (FRET) from PFP to FAM is inefficient when adding PFP into FAM-DNA/GO complex. If SP is added to FAM-DNA/GO complex, FAM-DNA is desorbed from GO surface due to the competitive binding of SP and FAM-DNA toward GO. In this case, FAM-DNA is close to PFP in the presence of PFP through strong electrostatic interaction, leading to the occurrence of efficient FRET. Based on the above phenomenon, we demonstrate a method to amplify fluorescence signal of traditional GO-based SP assay by introducing PFP. In comparison to the use of single GO, the combination of PFP with GO-based strategy displays high turn-on ratio and enhanced sensitivity with a limit of detection as low as 73 ng mL(-1) for SP detection. Satisfactory results in practical samples are also obtained by the recovery experiments, demonstrating the potential application of cationic conjugated polymer in plant-derived small molecule. (C) 2018 Elsevier B.V. All rights reserved.