▎ 摘　　要

Detection of applied antibiotics in food productions could be the first step to avoid future health consequences. The aim of this work was to the synthesis of molecularly imprinted polymer (MIP) particles via non-covalent procedure and evaluation of efficiency MIP for the selective collection of Cloxacillin (CLO) from aqueous and biological samples and consequently quantification of separated CLO using an electrochemical nanosensor. The effect of operational parameters including pH, contact time and MIP dosage for optimization of CLO collection condition were studied. The CLO quantities were determined by a developed electrochemical nanosensor based on a screen-printed electrode modified with graphene oxide and gold nanoparticles. The results showed that the optimum conditions for removal of CLO (92%) were determined at pH = 8.5, with 89 min as contact time and 0.79 g MIP. The linear range was from 110 to 750 nM and the detection limit of the nanosensor was 36 nM. The performance of the MIP-based nanosensor for the spiked Cloxacillin detection in real milk samples showed the potential of the developed nanosensor to be considered in future real sample measurement analysis. (C) 2019 Elsevier Ltd. All rights reserved.