• 文献标题:   Graphene Oxide as a Matrix for the Immobilization of Glucose Oxidase
  • 文献类型:   Article
  • 作  者:   ZHOU LY, JIANG YJ, GAO J, ZHAO XQ, MA L
  • 作者关键词:   graphene oxide, glucose oxidase, electrostatic attraction, immobilization, stability
  • 出版物名称:   APPLIED BIOCHEMISTRY BIOTECHNOLOGY
  • ISSN:   0273-2289
  • 通讯作者地址:   Hebei Univ Technol
  • 被引频次:   21
  • DOI:   10.1007/s12010-012-9884-4
  • 出版年:   2012

▎ 摘  要

The adsorption of glucose oxidase (GOD) on graphene oxide (GO) nanoparticles without using any cross-linking reagents and/or additional surface modification was studied. Results of Fourier-transform infrared and ultraviolet-visible absorption spectroscopy confirmed that GOD was successfully immobilized on GO surface. The obtained immobilized GOD showed a wide range of pH stability and improved thermal and storage stability. In addition, GO exhibited good biocompatibility, which has potential advantages for biomedical and clinical diagnosis applications.