▎ 摘　　要

An electrochemical sensor was fabricated for determination of cholesterol (ChO) based on the molecularly imprinting and signal amplification of graphene. The sensor was prepared by electropolymerizing phenol on the surface of graphene modified glassy carbon electrode with ChO as template. The structure, properties and molecular imprinting effect of the imprinted membrane were studied by SEM, CV and DPV. The results showed that the sensor had good selectivity and high sensitivity to cholesterol. The calibration graph for the determination of cholesterol was linear in the range of 8. 0. 10(-8) - 2. 0x10(- 4) mol/L, with the detection limit (S/N = 3) of 5. 6x10(-8) mol/L. The sensor was applied to the determination of cholesterol in human serum samples with satisfactory results.