▎ 摘　　要

From thelast two decades, the microalgal applications in sustainable biodiesel production is gaining attention due to the diminution of fossil fuels and rising fuel prices. There are different types of microalgae species have been characterized for lipid production under different nutrient starvation and supplementation condition. Herein, the indigenous microalga Monoraphidium sp. VV1 isolate was used for higher lipid accumulation. To propagate the biomass and biodiesel production, the microalgal biomass was improved with food waste hydrolysates (FWH) added in various concentrations (0.5%, 1%, 2%, and 4%), among that 2% of food waste showed a prominent biomass production than the control. Further, to augment the lipid accumulation in Monoraphidium sp. VV1, diverse stress conditions like methanol and graphene oxide (GO) nanosheets along with nitrogen starvation 0.1% iron (N- with FeCl3+) were provided in the culture medium. The lipid accumulation during N- with FeCl3+ is 29.96%, 4% of methanol stress yielded 28.1 mg/L/Day biomass productivity, and 39.5% of lipid content attained from 1% GO supplementation which are higher than the control (25.5%). Thus, considering Monoraphidium sp. VV1 growth and lipid yield at different conditions this microalga can be used as a feedstock for biofuel production for future energy demand.