• 文献标题:   Graphene oxide and GST-omega enzyme: An interaction that affects arsenic metabolism in the shrimp Litopenaeus vannamei
  • 文献类型:   Article
  • 作  者:   JOSENDE ME, NUNES SM, LOBATO RDO, GONZALEZDURRUTHY M, KIST LW, BOGO MR, WASIELESKY W, SAHOO S, NASCIMENTO JP, FURTADO CA, FATTORINI D, REGOLI F, MACHADO K, WERHLI AV, MONSERRAT JM, VENTURALIMA J
  • 作者关键词:   nanotoxicology, gstomega enzyme, arsenic metabolisation, molecular docking simulation
  • 出版物名称:   SCIENCE OF THE TOTAL ENVIRONMENT
  • ISSN:   0048-9697 EI 1879-1026
  • 通讯作者地址:   Univ Fed Rio Grande FURG
  • 被引频次:   2
  • DOI:   10.1016/j.scitotenv.2020.136893
  • 出版年:   2020

▎ 摘  要

Arsenic (As) is one of the most widespread contaminants; it is found in almost every environment. Its toxic effects on living organisms have been studied for decades, but the interaction of this metalloid with other contaminants is still relatively unknown, mainly whether this interaction occurs with emerging contaminants such as nanomaterials. To examine this relationship, the marine shrimp Litopenaeus vannamei was exposed for 48 h to As, graphene oxide (GO; two different concentrations) or a combination of both, and gills, hepatopancreas and musde tissues were sampled. Glutathione S-transferase (GST)-omega gene expression and activity were assessed. As accumulation and speciation (metabolisation capacity) were also examined. Finally, a molecular docking simulation was performed to verify the possible interaction between the nanomaterial and GST-omega. The main finding was that GO modulated the As toxic effect: it decreased GST-omega activity, a consequence related to altered As accumulation and metabolism. Besides, the molecular docking simulation confirmed the capacity of GO to interact with the enzyme structure, which also can be related to the decreased GST-omega activity and subsequently to the altered As accumulation and metabolisation pattern. (C) 2020 Elsevier B.V. All rights reserved.