• 文献标题:   Size-Exclusive Magnetic Graphene/Mesoporous Silica Composites with Titanium(IV)-Immobilized Pore Walls for Selective Enrichment of Endogenous Phosphorylated Peptides
  • 文献类型:   Article
  • 作  者:   SUN NR, DENG CH, LI Y, ZHANG XM
  • 作者关键词:   magnetic graphene, mesoporous silica, sizeexclusive, titanium, endogenous phosphorylated peptide
  • 出版物名称:   ACS APPLIED MATERIALS INTERFACES
  • ISSN:   1944-8244
  • 通讯作者地址:   Fudan Univ
  • 被引频次:   54
  • DOI:   10.1021/am502529a
  • 出版年:   2014

▎ 摘  要

Developing an effective separation method is necessary for identifying low-abundant endogenous phosphorylated peptides with the removal of proteins. In this work, we prepared size-exclusive magnetic graphene/mesoporous silica composites with titanium(lV)-immobilized pore walls (denoted as Ti4+-MGMSs) for capturing endogenous phosphorylated peptides for mass spectrometry analysis. The introduction of hydrophilic polydopamine simplified the synthetic process of Ti4+-MGMSs, and the ordered mesoporous channels are beneficial to the trapping of endogenous phosphopeptides while large-size proteins are excluded. Furthermore, the magnetic performance greatly simplifies the entire process of enrichment. With all of the advances, the novel Ti4+-MGMSs present high enrichment efficiency either from the low concentration of beta-casein tryptic digest (0.5 fmol/mu L) or the mixture of beta-casein tryptic digest and alpha-casein (or plus bovine serum albumin, with a mass ratio up to 1:500). Besides, Ti4+-MGMSs have also been successfully applied to enrich endogenous phosphorylated peptides from human serum and human saliva.