▎ 摘　　要

By using fluorophore (FAM) labeled aptamers as recognition elements and graphene oxide as a quencher, a highly selective and sensitive sensor was constructed for the fast and accurate detection of insulin. After the aptamer binding with graphene oxide, fluorescence will be quenched and fluorescence intensity disappeared; after addition of insulin, the solution fluorescence was restored. Based on the fact, we established a method for the determination of insulin concentration by measuring the fluorescence intensity. The results showed that the concentration of insulin in the range of 5 x 10(-8) - 1 x 10(-5) mol/L had a good linear relationship with the fluorescence intensity. The detection limit was 10 nmol/L. This method of detecting insulin had obvious advantages of fast detection speed, high selectivity and low detection limit.