• 文献标题:   RETRACTED: Ionic liquid-functionalized graphene for fabricating an amperometric acetylcholinesterase biosensor (Retracted article. See vol. 138, pg. 7422, 2013)
  • 文献类型:   Article, Retracted Publication
  • 作  者:   LI YP, HAN GY
  • 作者关键词:  
  • 出版物名称:   ANALYST
  • ISSN:   0003-2654 EI 1364-5528
  • 通讯作者地址:   Shanxi Univ
  • 被引频次:   17
  • DOI:   10.1039/c2an35065c
  • 出版年:   2012

▎ 摘  要

This work reports a sensitive amperometric biosensor for organophosphate pesticides (OPs) fabricated by modifying a glassy carbon electrode with acetylcholinesterase (AChE) immobilized on ionic lwiquid-functionalized graphene (IL-G). The functionalized graphene sheets had good dispersibility and longterm stability in various solvents. The as-prepared biosensor showed high affinity to acetylthiocholine (ATCl) with a Michaelis-Menten constant (K-m) value of 0.77 mM. Furthermore, based on the inhibition by OPs of the enzymatic activity of the immobilized AChE, and using carbaryl as a model compound, the inhibition of carbaryl was proportional to its concentration ranging from 0.0025 to 0.48 and 0.48 to 1.42 mu g mL(-1) with a detection limit of 0.8 ng mL(-1) (S/N = 3). The developed biosensor exhibited a good performance for OPs detection, including good reproducibility and acceptable stability, which provided a new and promising tool for the analysis of enzyme inhibitors.