▎ 摘　　要

A novel Pt/RTIL/graphene hybrid nanocomposite was synthesized via a simple one-step microwave method. The added RTIL served as a stabilizer to prevent the aggregation of both graphene and Pt particles. In this work, four imidazolium-based RTILs with different functional groups were involved in the preparation of Pt/RTIL/graphene nanocomposites. The influence of the functional groups of the synthesized RTILs on the distribution and electrochemical properties of the hybrid nanocomposites was systematically investigated by X-ray diffraction (XRD), transmission electron microscopy (TEM) and linear sweep voltammetry (LSV). What is more, combined with the glucose oxidase, a glucose biosensor based upon the Pt/RTIL/graphene was fabricated accordingly, which displayed an excellent response to glucose at a potential of -150 mV with a good linear range for glucose from 1.000 uM to 1.164 mM. The detection limit was calculated to be 0.8930 mu M (S/N = 3), accompanied with a favorable reproducibility and storage stability. Under the optimal conditions, the constructed glucose biosensor was successfully applied to the determination of glucose concentrations in real serum samples. This method herein has proved to be sensitive and reproducible, which enables its promising application in physiology and pathology. (C) 2013 Elsevier B.V. All rights reserved.