▎ 摘　　要

This study describes an amperometric sensor for hydrogen peroxide (H2O2) that uses an ITO glass electrode which was modified with a nanocomposite consisting of electrochemically reduced graphene oxide and gold nanoclusters (AuNCs). The sensor was used to quantify extracellular H2O2 released from human neuroblastoma cells of type SH-SY5Y. The calibration plot, established best at a working voltage of -0.4 V (vs. Ag/AgCl) is linear in the 40 nmolai...L-1 to 2 mu molai...L-1 concentration range, and the detection limit is 20 nmolai...L-1 (at a signal-to-noise ratio of 3). The method was further applied to study bupivacaine-induced cell damage and the protective effects of alpha-lipoic acid. The study indicated that pretreatment of the cells with lipoic acid retards cell damage induced by bupivacaine. The sensor can be easily fabricated, is disposable and highly sensitive. The sensor is perceived to represent an alternative for studying the interactions of drugs with cells, and as an effective tool to quantify cell-secreted H2O2.