▎ 摘　　要

Quantitative detection of antibiotic residues in animal food stuffs is of great significance. In this work, a highly sensitive electrochemical aptasensor for the sensitive detection of streptomycin antibiotic was fabricated based on a novel signal amplification strategy. Specifically, this aptasensor was constructed utilizing porous carbon nanorods (PCNR) formed by porous carbon nanosphere and multifunctional graphene composite (GR-Fe3O4-AuNPs) as biosensing substrate. PCNR samples with large specific pore volume and high specific surface area were successfully prepared by hydrothermal and chemical activation treatment for the first time. GR-Fe3O4-AuNPs was served as labels to achieve a high sensitivity and low limit of detection (LOD). Under the optimized conditions, the proposed aptasensor exhibited a high sensitivity and a wider linearity to streptomycin in the range 0.05-200 ng/mL with a low detection limit of 0.028 ng/mL. The proposed aptasensor displayed an excellent analytical performance with great reproducibility, high selectivity and stability. In addition, the as-prepared aptasensor was successfully utilized for the determination of streptomycin in real samples. (C) 2016 Elsevier B.V. All rights reserved.