• 文献标题:   3,4,9,10-Perylenetetracarboxylic dianhydride functionalized graphene sheet as labels for ultrasensitive electrochemiluminescent detection of thrombin
  • 文献类型:   Article
  • 作  者:   GAN XX, YUAN R, CHAI YQ, YUAN YL, CAO YL, LIAO YH, LIU HJ
  • 作者关键词:   3 4 9 10perylenetetracarboxylic dianhydride, grapheme sheet, electrochemiluminescence, peroxydisulfate, sandwichtype
  • 出版物名称:   ANALYTICA CHIMICA ACTA
  • ISSN:   0003-2670
  • 通讯作者地址:   Southwest Univ
  • 被引频次:   21
  • DOI:   10.1016/j.aca.2012.03.007
  • 出版年:   2012

▎ 摘  要

A novel tracer, 3,4,9,10-perylenetetracarboxylic dianhydride(PTCDA)functionalized graphene sheet (GS) composite (GS-TCDA). is employed to label the secondary anti-thrombin aptamer (TBA) to construct an ultrasensitive electrochemiluminescent sandwich-type aptasensor. The GS provided large surface area for loading abundant PTCDA and TBA with good stability and biocompatibility. Because of the excellent electroconductivity of GS and the desirable optical properties of PTCDA, the as-formed Apt II bioconjugate considerably amplified the electrochmiluminescence (ECL) signal of peroxydisulfate (S2O82-) and worked as the desirable label for Apt II. On the basis of the considerably amplified ECL signal and sandwich format, an extremely wide range from 1 fM to 1 nM with an ultralow detection limit of 0.33 fM for thrombin was obtained. Additionally, the selectivity and stability of the proposed aptasensor were also excellent. Thus, this procedure has great promise for detection of thrombin present at ultra-trace levels during early stage of diseases. (c) 2012 Elsevier B.V. All rights reserved.