▎ 摘　　要

An electrochemical biosensor was developed using Pt-nanoparticles (Pt-NPs) dispersed graphene based on a boron-doped diamond thin film electrode. To compare its performances with those of other biosensors, glucose was used as a target analyte. This biosensor exhibited a wide linear range, a low detection limit and a higher sensitivity compared to other amperometric biosensors using graphene-based electrodes. In addition, the biosensor promotes a direct electron transfer between the redox enzymes and the electrode surface and detects low concentration analytes. The excellent performance of the biosensor is attributed to the synergistic effect of the Pt-NPs, graphene sheet and the BDD thin film. Therefore, it can be a promising application for electrochemical detection of analytes.