• 文献标题:   Myoglobin-silver reduced graphene oxide nanocomposite stochastic biosensor for the determination of luteinizing hormone and follicle-stimulating hormone from saliva samples
  • 文献类型:   Article
  • 作  者:   GUGOASA LAD, STEFANVAN STADEN RI, VAN STADEN JF, COROS M, PRUNEANU SM
  • 作者关键词:   luteinizing hormone, folliclestimulating hormone, stochastic biosensor, reduced graphene oxide, silver nanoparticle
  • 出版物名称:   ANALYTICAL BIOANALYTICAL CHEMISTRY
  • ISSN:   1618-2642 EI 1618-2650
  • 通讯作者地址:   Natl Inst Res Dev Electrochem Condensed Matte
  • 被引频次:   0
  • DOI:   10.1007/s00216-020-02663-z EA MAY 2020
  • 出版年:   2020

▎ 摘  要

A silver reduced graphene oxide (Ag-rGO) nanocomposite paste was prepared for the assay of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in children's saliva. The paste was modified with a solution of myoglobin (Myb) to form the stochastic biosensor, to improve the sensitivity of the method. The Ag-rGO powder and Myb-Ag-rGO paste were morphologically and structurally characterized by SEM, TEM and XRD measurements. For the molecular recognition of the hormones, the biosensor based on Myb-Ag-rGO reached determination limits of 8.5 x 10(-3) UI L-1 (2.0 x 10(-12) g/mL) for luteinizing hormone and 1.4 x 10(-2) UI L-1 (1.0 x 10(-12) g/mL) for follicle-stimulating hormone. The determination of both hormones in saliva samples collected from children was done with high reliability. Graphical