▎ 摘 要
A silver reduced graphene oxide (Ag-rGO) nanocomposite paste was prepared for the assay of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in children's saliva. The paste was modified with a solution of myoglobin (Myb) to form the stochastic biosensor, to improve the sensitivity of the method. The Ag-rGO powder and Myb-Ag-rGO paste were morphologically and structurally characterized by SEM, TEM and XRD measurements. For the molecular recognition of the hormones, the biosensor based on Myb-Ag-rGO reached determination limits of 8.5 x 10(-3) UI L-1 (2.0 x 10(-12) g/mL) for luteinizing hormone and 1.4 x 10(-2) UI L-1 (1.0 x 10(-12) g/mL) for follicle-stimulating hormone. The determination of both hormones in saliva samples collected from children was done with high reliability. Graphical