▎ 摘　　要

Electrochemiluminescence (ECL) of ruthenium complexes has broad applications and the immobilization of Ru(bpy)(3)(2+) has received extensive attention. In comparison with Ru(bpy)(3)(2+), Ru(phen)(3)(2+) can be immobilized more easily because of its better adsorbability. In this study, immobilization of Ru(phen)(3)(2+) for ECL analysis has been demonstrated for the first time by using graphene oxide (GO) as an immobilization matrix. The immobilization of Ru(phen)(3)(2+) is achieved easily by mixing Ru(phen)(3)(2+) with GO without using any ion exchange polymer or covalent method. The strong binding of Ru(phen)(3)(2+) with GO is attributed to both the pi-pi stacking interaction and the electrostatic interaction. The Ru(phen)(3)(2+)/GO modified electrode was characterized by using tripropylamine (TPA) as the coreactant. The linear range of TPA is from 3 x 10(-7) to 3 x 10(-2) mol L-1 with the detection limit of 3 x 10(-7) mol L-1. The ECL sensor demonstrates outstanding long-term stability. After the storage in the ambient environment for 90 days, the ECL response remains comparable with its original signal. (C) 2012 Elsevier B.V. All rights reserved.