▎ 摘　　要

In this work we report the synthesis of graphene oxide - horseradish peroxidase biocomposite (GO-HRP) by a solution method without using any cross-linkers. Then, the GO-HRP biocomposite was drop casted on a screen printed carbon electrode (SPCE) followed by the electrochemical reduction to reduced graphene oxide-HRP (ERGO-HRP) composite. The ERGO-HRP modified SPCE showed good electroanalytical properties towards the amperometric determination of H2O2. The film has been characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Both the studies revealed the effective immobilization of HRP on ERGO. The SPCE/ERGO-HRP showed a linear range of detection of 9 - 195 mu M. The electrode also detects H2O2 in real sample solutions with appreciable accuracy.