• 文献标题:   A Label-free Cardiac Troponin T Electrochemiluminescence Immunosensor Enhanced by Graphene Nanoplatelets
  • 文献类型:   Article
  • 作  者:   AZAM NFN, MOHAMMAD NA, LIM SA, AHMED MU
  • 作者关键词:   graphene nanoplatelet, screenprinted electrode, electrochemiluminescence, ctnt detection
  • 出版物名称:   ANALYTICAL SCIENCES
  • ISSN:   0910-6340 EI 1348-2246
  • 通讯作者地址:   Univ Brunei Darussalam
  • 被引频次:   3
  • DOI:   10.2116/analsci.19P105
  • 出版年:   2019

▎ 摘  要

In this study, a direct and label-free immunosensor was designed and constructed by modifying the screen-printed electrode with graphene nanoplatelets (GNPs) for the detection of the cardiac troponin T (cTnT). Firstly, GNPs were drop-casted onto carbon working electrode. Monoclonal cTnT antibodies were then immobilized on the GNPs via physical adsorption; finally, BSA was introduced to block non-specific binding sites. The detection of cTnT was performed using an electrochemiluminescence (ECL) technique with tris(bipyridine)ruthenium(II) chloride ([Ru(bpy)(3)]Cl-2) used as a luminophore and TPrA (tripropylamine) as a co-reactant. The ECL intensity was demonstrated to be directly proportional to the cTnT concentration where a linear range from 100 pg mL(-1) to 5 fg mL(-1) of the cTnT detection was established. An extremely low limit of detection was achieved to be 0.05 fg mL(-1) with an outstanding specificity. Additionally, this immunosensor showed excellent percentage recovery for real samples analyses in artificially spiked human serum.