▎ 摘　　要

Designing a highly sensitive, fast response, cheap, and flexible biosensing platform has important significance in the diagnosis of diabetes. Herein, we developed an ultra-fast and sensitive glucose sensor based on CuO nanoflowers-coated stereo-graphene electrode on carbon cloth. The three-dimensional graphene (3DG) nanostructures were established on the carbon cloth (CC) by radio frequency plasma enhanced chemical vapor deposition (RF-PECVD), and CuO nanoflowers subsequently obtained via chemical deposition. The prepared CuO@3DG@CC electrode with self-assembled structures provided abundant ion diffusion channels, large specific surface area and high conductivity, which shows an ultrasensitive current response to glucose. The detection limit of the sensor is as low as 0.068 mu M and the response efficiency is as fast as 0.5 s. The electrode has two linear ranges with 0.5-115.5 mu M and 165.5-1165.5 mu M and has a strong anti-interference ability and shows an excellent stability. This kind of flexible enzyme-free glucose sensor has been successfully applied to the detection of glucose in actual serum samples and shows a good accuracy, which is likely be applied to the medical testing field and the commercial production and has potential to further applied in wearable sensors.