• 文献标题:   Development of a Novel Quantum Dots and Graphene Oxide Based FRET Assay for Rapid Detection of invA Gene of Salmonella
  • 文献类型:   Article
  • 作  者:   GUO JBA, CHAN EWC, CHEN S, ZENG ZL
  • 作者关键词:   salmonella, inva, quantum dot, graphene oxide, fret assay, rapid detection
  • 出版物名称:   FRONTIERS IN MICROBIOLOGY
  • ISSN:   1664-302X
  • 通讯作者地址:   South China Agr Univ
  • 被引频次:   3
  • DOI:   10.3389/fmicb.2017.00008
  • 出版年:   2017

▎ 摘  要

A novel, rapid and simple fluorescence resonance energy transfer (FRET) based Salmonella specific gene, invA, detection system was developed, in which quantum dots (QDs) and graphene oxide (GO) worked as fluorescent donor and quencher, respectively. By measuring the fluorescence intensity signal, the Salmonella specific invA gene could be sensitively and specifically detected with a limit of detection (LOD) of similar to 4 nM of the invA gene in 20 min. The developed system has the potential to be used for Salmonella detection in food and environmental samples and further developed into a platform for detection of other bacterial pathogens.