▎ 摘　　要

Recently, using quorum-sensing inhibitors to degrade signaling molecules and mitigating membrane's biofouling is a new breakthrough. In this study, a new graphene oxide@acylase (GO@AC) nanoparticle was prepared via adsorption method. GO, GO@AC and Polyvinyl pyrrolidone (PVP) were added to a poly(vinylidene fluoride) (PVDF) membrane casting solution by embedding and blending, respectively, and the nanohybrid membranes were prepared through phase inversion by the immersion precipitation technique. The results showed that the biological activity of PVDF/GO@AC/PVP membrane could be maintained for about 28-30 days. To examine the antibiofouling characteristics of nanohybrid membranes, sterilized nanohybrid membranes were immersed in a Pseudomonas aeruginosa nutrient solution for 120 h, and the three-dimensional structures of biofilm were observed by a confocal laser scanning microscope. The results showed that the biofilm thickness of PVDF/PVP, PVDF/GO/PVP and PVDF/GO@AC/PVP membrane was about 60, 25 and 40 mu m, respectively; the biomass proportion of the latter membrane was 3.04 mu m(3)/mu m(2), which was only 16% of the PVDF membrane biomass. This showed that the new PVDF/GO@AC/PVP membrane had better inhibitory effect on biofilm formation, and this study provided a new way to mitigate the membrane's biofouling.