▎ 摘　　要

As a short peptide containing active thiol group, glutathione participates in many intracellular reactions, so it is of great significance to detect glutathione in cells. In this work, amino-rich graphene quantum dots were synthesized, which could coordinate with copper ions and yield aggregation-induced fluorescence quenching. However, copper ions and glutathione have stronger coordination ability, so that glutathione can promote the dissociation of copper ions from graphene quantum dots, and then lead to the fluorescence recovery of graphene quantum dots. In BR buffer media at pH 6.8, glutathione can gradually recover the fluorescence of graphene quantum dots (1 mu g/mL) that is quenched by Cu2+ (250 mu mol/L), which can finish within 20 min. Additionally, recovery degree of fluorescence signal is linear to the concentration of glutathione in the range of 20-500 mu mol/L, and the detection limit is 3.4 mu mol/L. This method is applicable to the detection of glutathione in cell lysate by using the switch action of copper ion to improve the selectivity.