▎ 摘　　要

This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on three-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin-coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca2+ fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 pm. These findings will further the development of advanced three dimensional graphene platforms for therapeutic applications and tissue engineering.