• 文献标题:   Adenylate kinase immobilized on graphene oxide impairs progression of human lung carcinoma epithelial cells through adenosinergic pathway
  • 文献类型:   Article, Early Access
  • 作  者:   ANNA H, BARTOSZ S, JOANNA C, TOMASZ R, MAREK W, MAGDALENA W, KATARZYNA R
  • 作者关键词:   adenosine, adenylate kinase, anticancer activity, graphene oxide, immobilization, purinergic signaling
  • 出版物名称:   JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
  • ISSN:   1549-3296 EI 1552-4965
  • 通讯作者地址:  
  • 被引频次:   0
  • DOI:   10.1002/jbm.a.37555 EA MAY 2023
  • 出版年:   2023

▎ 摘  要

Purinergic signaling, the oldest evolutionary transmitter system, has been increasingly studied as a pivotal target for novel anti-cancer therapies. In the present work, the developed nanobiocatalytic system consisting of adenylate kinase immobilized on graphene oxide (AK-GO) was characterized in terms of its physicochemical and biochemical properties. We put special emphasis on the AK-GO influence on purinergic signaling components, that is, ecto-nucleotides concentration and ecto-enzymes expression and activity in human lung carcinoma epithelial (A549) cells. The immobilization-dependent modification of AK kinetic parameters allowed for the removal of ATP excess while maintaining low ATP concentrations, efficient decrease in adenosine concentration, and control of the nucleotide balance in carcinoma cells. The cyto- and hemocompatibility of developed AK-GO nanobiocatalytic system indicates that it can be successfully harnessed for biomedical applications. In A549 cells treated with AK-GO nanobiocatalytic system, the significantly decreased adenosinergic signaling results in reduction of the proliferation and migration capability of cancer cells. This finding is particularly relevant in regard to AK-GO prospective anti-cancer applications.