▎ 摘　　要

The present work reported the one-pot preparation of silver nanoparticles doped with graphene oxide (Ag@GO), using glucose as an eco-friendly reducing agent. The effects of synthesis conditions on the introduction of AgNPs onto graphene oxide sheets were confirmed by modern analytical techniques and antibacterial assays. Results showed that the Ag@GO-4 sample prepared with glucose:AgNO3:GO mass ratio of 1:1:1 at 60 degrees C for 60 min exhibited an appropriate one for further studies with AgNPs sizing of 16.66 +/- 3.73 nm. The Ag@GO-4 effectively inhibited the development of S. enterica, P. aeruginosa, and S. aureus with the 50 % inhibitory concentration at 14.5, 0.9, and 6.8 mu g/mL, respectively. The simultaneous effects of Ag@GO concentration, pH, interaction time, annealing temperature, and bacterial density on the antibacterial activity of Ag@GO-4 were also investigated by Plackett-Burman and Box-Behnken design. The optimal values deducing for Ag@GO-4 concentration, pH, and bacterial density were 40 mu g/mL, 5.5, and 5.1 x 10(6) CFU/mL, respectively, which could impressively reach 99.99 % of S. enterica elimination in practice. The interaction time and annealing temperature implied a negligible effect on the antibacterial activity of Ag@GO-4. All results of the present study affirmed the future use of Ag@GO not only as an efficient antibacterial agent but also potentially as a novel anti-virus method of treatment. (C) 2021 Elsevier Ltd. All rights reserved.