▎ 摘　　要

A simple electrochemical deposition method for the immobilization of protein-conjugated graphene onto an electrode has been developed. A unique one-step electrochemical deposition of cytochrome c (Cyt c) conjugated with electrochemically reduced graphene oxide (ErGO) onto the surface of glassy carbon electrode (GCE) without any cross-linkers and the direct electron transfer between Cyt c and the electrode was obtained by the Cyt c-ErGO-modified GCE. The characteristics of the ErGO-Cyt c-modified GCE were compared with those of an ErGO-modified GCE without Cyt c. The direct electron transfer rate constant (k(et)) was found to be 6.2/s. This approach provides an avenue for the immobilization of various proteins and enzymes with ErGO and could be expanded for diverse biosensor applications.