• 文献标题:   Three-Dimensional Quantitative Intracellular Visualization of Graphene Oxide Nanoparticles by Tomographic Flow Cytometry
  • 文献类型:   Article
  • 作  者:   PIRONE D, MUGNANO M, MEMMOLO P, MEROLA F, LAMA GC, CASTALDO R, MICCIO L, BIANCO V, GRILLI S, FERRARO P
  • 作者关键词:   nanographene oxide, cellular uptake, tomographic flow cytometry, 3d intracellular spatial distribution of nanoparticle, single cell 3d microscopy
  • 出版物名称:   NANO LETTERS
  • ISSN:   1530-6984 EI 1530-6992
  • 通讯作者地址:  
  • 被引频次:   19
  • DOI:   10.1021/acs.nanolett.1c00868 EA JUL 2021
  • 出版年:   2021

▎ 摘  要

Interaction of nanoparticles (NPs) with cells is of fundamental importance in biology and biomedical sciences. NPs can be taken up by cells, thus interacting with their intracellular elements, modifying the life cycle pathways, and possibly inducing death. Therefore, there is a great interest in understanding and visualizing the process of cellular uptake itself or even secondary effects, for example, toxicity. Nowadays, no method is reported yet in which 3D imaging of NPs distribution can be achieved for suspended cells in flow-cytometry. Here we show that, by means of label-free tomographic flow-cytometry, it is possible to obtain full 3D quantitative spatial distribution of nanographene oxide (nGO) inside each single flowing cell. This can allow the setting of a class of biomarkers that characterize the 3D spatial intracellular deployment of nGO or other NPs clusters, thus opening the route for quantitative descriptions to discover new insights in the realm of NP-cell interactions.