• 文献标题:   Ultrasensitive electrochemiluminescence immunosensor based on nanoporous gold electrode and Ru-AuNPs/graphene as signal labels
  • 文献类型:   Article
  • 作  者:   LI M, ZHANG M, GE SG, YAN M, YU JH, HUANG JD, LIU S
  • 作者关键词:   electrochemiluminescence, nanoporous gold, rugold nanoparticles/graphene composite, cancer antigen 125
  • 出版物名称:   SENSORS ACTUATORS BCHEMICAL
  • ISSN:   0925-4005
  • 通讯作者地址:   Univ Jinan
  • 被引频次:   18
  • DOI:   10.1016/j.snb.2013.01.042
  • 出版年:   2013

▎ 摘  要

In the paper, a simple and sensitive sandwich-type electrochemiluminescence (ECL) immunosensor was proposed for the detection of cancer antigen 125 (CA 125) on a nanoporous gold (NPG) modified glassy carbon electrode (GCE). The NPG was easily prepared by a selective dissolution of silver from silver/gold alloy in nitric acid. The fabrication of Ru(bpy)(3)(2+)-gold nanoparticles (Ru-AuNPs) aggregates was owing to the electrostatic interactions between citrate-capped AuNPs and Ru in aqueous medium. The Ru-AuNPs composite assembled with poly(diallyldimethylammonium chloride) functionalized graphene nanosheets (GR) (Ru-AuNPs/GR) was used as ECL labels, which has large surface area, good biocompatibility and electronic conductivity. The ECL intensity observed by the application of as-prepared Ru-AuNPs/GR composites was enhanced 6-fold compared to those of Ru-AuNPs. The primary antibody of CA 125 (Ab(1)) was first immobilized on the NPG modified electrode, then the antigen and the secondary antibody (Ab(2)) were conjugated to form a sandwich-type immunocomplex through the specific interaction. The proposed ECL immunosensor provided a wide linear response range over 0.01-100 U mL(-1) with a detection limit of 0.005 U mL(-1). The ECL immunosensor showed high sensitivity, good reproducibility, satisfied regeneration and selectivity, and may exhibit an attractive approach for other analyte determination. (C) 2013 Elsevier B.V. All rights reserved.