▎ 摘　　要

Protein attachment on the surface of nanomaterial is termed as protein corona formation. This protein corona has very high influence on the in vivo activity of nanomaterials. They alter the behaviour of nanomaterial. The interaction of nanomaterial with blood and cells changes due to this corona formation. The corona formation changes with time and protein concentration. It is very important to analyse the protein corona formation to utilize the nanomaterial for medical applications like drug delivery, drug targeting, imaging and tissue engi-neering. In this work we have prepared graphene oxide (GO) by hummers method and characterized it with X-Ray Diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR) and Raman spectroscopy. Bovine serum albumin (BSA) was interacted with GO to form the protein corona. The concentration of BSA was varied to get different GO/BSA samples. With increase in the BSA concentration the protein attachment on GO increases. This protein attachment on GO influences the antimicrobial property, hemocompatibility and cytocompatibility of GO.