▎ 摘　　要

In this paper, a novel optical probing strategy for the detection of amantadine has been successfully developed based on the competitive host-guest interaction of amantadine and rhodamine6G (R6G) with mono-[6-(2-aminoethylamino)-6-deoxy]-beta-cyclodextin (EDA-CD). We synthesized the EDA-CD and graphene oxide (GO), and the EDA-CD functionalized graphene oxide (GO-CD) was prepared by covalently linking amino group of the EDA-CD with carboxylic acid groups on graphene oxide surface. R6G was chosen as a fluorescent probe because of its capability to enter into the cavity of EDA-CD. After R6G was incorporated into the hydrophobic inner cavity of GO-CD, significant fluorescence quenching was observed due to the fluorescence resonance energy transfer (FRET) between R6G and GO-CD complex. When amantadine was added to the GO-CD/R6G complex system, R6G would be released from the inner cavity of GO-CD and result in the fluorescence recovery due to the higher affinity between amantadine and EDA-CD, which could be used as a fluorescent probe for the detection of amantadine. The proposed method was successfully applied to the determination of amantadine in pharmaceutical capsule with good accuracy and satisfactory recovery. (C) 2014 Elsevier B.V. All rights reserved.