▎ 摘　　要

An electrochemical H2O2 sensor was developed using an alginate/water-soluble prussian blue/reduced graphene oxide (A/s-PB/rGO) composite on carbon screen printed electrodes for use in bioapplications. Carbon screen printed working electrodes were modified with A/s-PB, A/rGO, and A/s-PB/rGO composite solutions by in situ gelation. The composites were characterized by Fourier transform infrared spectroscopy and field emission scanning electron microscopy. The cyclic voltammetric measurements made using the electrode modified by A/s-PB/rGOs showed improved electrochemical reaction and stability over the A/s-PB and A/rGO modified electrodes. A two-electron transfer process is indicated at the modified electrode surface. The oxidation current showed a detection limit of 4 mM (S/N = 3) and linear range of 4-28 mM. The A/s-PB/rGO modified electrode provides a tool for high-sensitivity (2.7 mu A.mM(-1).cm(-2)) and stable H2O2 analyses with a low limit of detection. Development of biosensor-based modified electrodes is important for biomedical and environmental applications.